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(@wingmankitchen) 分享的帖子 ·

This curiosity is debuting at Coachella: a puffle cone filled not with ice cream, but popcorn chicken. Otherwise known as the “Puff Daddy,” the latest way to consume chicken is a savory twist on a sweet dessert that took the internet by storm. Find that ish ASAP.

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(@sunnyblue_inc) 分享的帖子 ·

Location: VIP (near white peak tents)

Take a break from all that fried goodness, and instead, indulge in Sunny Blue’s gorgeously seasoned hand-crafted ombusubi and takoyaki. Any food that can be handheld and contains rice is an A+ is my book.

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Trapizzino (@trapizzino) 分享的帖子 ·

Get a taste of Rome at Trapizzino, where you can pick up fluffy dough pockets filled with anything from oxtail ragu to eggplant Parmesan. They’re rich, decadent, and neat – the mess-free solution to pizza on the go.

Fridays = meaty/cheesy goodness

HAYDEN (@haydenwinebar) 分享的帖子 ·

Location: VIP Rose Garden

Just because you’re in the middle of the desert doesn’t mean you can’t be your bougie self. Hayden, I kid you not, is serving wine and charcuterie so you don’t have to skip a beat. Treat yo’ self.

Behold the glory of a #HIHOcheeseburger!!

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(@hihocheeseburger) 分享的帖子 ·

Location: VIP (near the white peak tents)

Can you even believe there are 100% grass-fed Wagyu beef burgers at Coachella? Wagyu. Beef. The team behind sushi fan favorite Sugarfish has made its way out of the sea to bring you top-notch quality burgers.

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The Exchange (@theexchangerestaurant) 分享的帖子 ·

Festival dining isn’t all booth-food. With VIP access, you have the option to eat at one of three full-service, open-air pop up restaurants from Southern California. , a "world-class butcher shop;", Freeland LA’s take on Israeli-Californian cuisine; and , an all-vegan Mediterranean and California-inspired hot spot will each be offering prix-fixe menus. Reservations are available in advance via OpenTable.

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// ¿Descubren un nuevo continente?
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How synergy between Bcd and the terminal system is achieved for each target gene is not clear. One possibility is that the Torso phosphorylation cascade directly modifies the Bcd protein, increasing its potency as a transcriptional activator. Mutations in Bcd's MAP-kinase phosphorylation sites partially reduce the ability of Bcd to activate otd , consistent with this hypothesis ( 43 ). Alternatively, the terminal system has been shown to repress the activities of ubiquitously expressed repressor proteins (reviewed in ref. Cheap In China 2018 Discount Marc Jacobs Mohair MidRise Pants Deals Cheap Price Sale Explore NJ03Mkr
). Perhaps repression by the terminal system creates posterior to anterior gradients of these proteins, which then compete with Bcd-dependent activation mechanisms to establish posterior boundaries of target gene expression.

Interactions between Bcd, maternal Hb, and the terminal system may be critical for the initial positioning of target gene expression patterns, but it is clear that other layers of regulation are required for creating the correct order of gene expression boundaries in the anterior part of the early embryo. Almost all known Bcd target genes are transcription factors, and there is evidence that they regulate each other by feed-forward activation and repression mechanisms. Each target gene contains one or more CRMs, each of which is composed of a specific combination and arrangement (code) of transcription factor binding sites (e.g., refs. Red Valentino Embroidered Silk Top Pick A Best Sale Shop For Discount Codes Clearance Store Free Shipping View 9C1H1dM
and 46 ). Unraveling the mechanisms that differentially position Bcd target will require the detailed dissections of CRMs that direct spatially distinct expression patterns.

The maternal-effect alleles used in this study were vas PD , exu PJ , and bcd E1 . mRNA and protein staining patterns were assayed using standard techniques. Fluorescent expression data were collected by confocal microscopy and analyzed using MATLAB (Mathworks). For microarray analysis, we isolated total RNA from 2–4 h embryos lacking bcd (0B; from bcd E1 females), and 1B, 2B, and 6B vas exu embryos, and hybridized cDNA made from each genetic background to whole-genome Drosophila tiling arrays from Affymetrix. We then performed statistical analyses to identify a set of 3,600 genes that varied significantly when Bcd levels were changed. Genes were ranked in terms of the fold change of their expression for all pairs of mutants: 0B–1B vas exu , 0B–2B vas exu , and 0B–6B vas exu . Complete methods are described in SI Text .

Acknowledgments

We thank Jerry Huang and Hongtao Chen for providing Bcd cluster data, Alison Mello for assistance with microarray data analysis, Huey Ling Kao for assistance in MATLAB programming, Gary Struhl for the HB0.8 LacZ strain, Claude Desplan for helpful comments on the manuscript, Yuri Goltsev and Uli Loehr for sharing data before publication, and Gozde Yucel for stimulating discussions. This work was supported by National Institutes of Health Grant GM 51946 in a facility constructed with support from Research Facilities Improvement Grant C06 RR-15518–01 from the National Center for Research Resources, National Institutes of Health.

Footnotes

Author contributions: A.O.-E. and S.S. designed research; A.O.-E. and D.Y. performed research; A.O.-E. and P.S. contributed new reagents/analytic tools; A.O.-E., D.Y., and A.T. analyzed data; and A.O.-E., P.S., and S.S. wrote the paper.

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